2025 (Current Year) Faculty Courses School of Life Science and Technology Undergraduate major in Life Science and Technology
Instrumental Analysis in Bioscience
- Academic unit or major
- Undergraduate major in Life Science and Technology
- Instructor(s)
- Toshiaki Kamachi / Satoshi Murakami / Yoshitaka Ishii / Toshinori Fujie / Nobuhiro Hayashi / Kayo Nozawa / Tetsuya Kadonosono / Hiroshi Tsutsumi / Takao Yasui / Mako Kamiya / Kiyohiko Kawai / Asako Yamayoshi
- Class Format
- Lecture (Face-to-face)
- Media-enhanced courses
- -
- Day of week/Period
(Classrooms) - 3-4 Tue (M-278(H121)) / 3-4 Fri (M-278(H121))
- Class
- -
- Course Code
- LST.A242
- Number of credits
- 200
- Course offered
- 2025
- Offered quarter
- 3Q
- Syllabus updated
- Oct 2, 2025
- Language
- Japanese
Syllabus
Course overview and goals
This course focuses on instrumental analysis, including fluorescence spectroscopy, circular dichroism, NMR, ESR, microscopy, mass spectrometry, chromatography, electrophoresis, flow cytometry and their data treatment. Theory and practical approaches of these experimental methods are given.
Course description and aims
By the end of this course, students will be able to understand :
1) Spectroscopic technique used in biological experiments
2) Chromatography
3) Electrophoresis
4) Microscopy
5) Data analysis
4) Metal ions analysis
5) Computational biochemistry
Keywords
Instrumental analysis, Spectroscopy, Microscopy, Chromatography, Flow cytometry
Competencies
- Specialist skills
- Intercultural skills
- Communication skills
- Critical thinking skills
- Practical and/or problem-solving skills
Class flow
At the beginning of each class, Instrumental Analysis in Bioscience is reviewed. At the end of every classes, students' understanding are accessed by exams.
Course schedule/Objectives
| Course schedule | Objectives | |
|---|---|---|
| Class 1 | Chromatography |
Theory and analytical applications of chromatography |
| Class 2 | Single-molecule Spectroscopy |
Theory and analytical applications of single molecule spectroscopy |
| Class 3 | Electrophoresis |
Theory and analytical applications of NMR & IR spectra |
| Class 4 | NMR and IR spectra |
Theory and analytical applications of Flow cytometry |
| Class 5 | Theory of magnetic resonance and ESR |
Theory of magnetic resonance and electron paramagnetic resonance |
| Class 6 | Ultra violet/ visible spectroscopy |
Theory and applications of UV/Vis spectroscopy |
| Class 7 | Fluorescence spectroscopy |
Theory and analytical applications of fluorescence spectroscopy |
| Class 8 | Flow cytometry Mass spectrometry |
Theory and analytical applications of mass spectrometry |
| Class 9 | Theory of errors, PCR(Digital PCR&qPCR), Next-Generation Sequencer |
Theory of errors, Theory and analytical applications of PCR(Digital PCR&qPCR), Next-Generation Sequencer |
| Class 10 | Flow cytometry |
Theory and analytical of Flow cytometry |
| Class 11 | Circular dichroism |
Theory and analytical applications of circular dichroism |
| Class 12 | Immunochemical analysis |
Theory and analytical applications of immunochemical analysis |
| Class 13 | Electron microscopy |
Theory and analytical applications of electron microscopy |
| Class 14 | X-ray analysis |
Theory and analytical applications of X-ray analysis |
Study advice (preparation and review)
To enhance effective learning, students are encouraged to spend approximately 100 minutes preparing for class and another 100 minutes reviewing class content afterwards (including assignments) for each class.
They should do so by referring to textbooks and other course material.
Textbook(s)
ISBN：978-4-06-139796-5
Reference books, course materials, etc.
ISBN-13: 978-4807906956
Evaluation methods and criteria
At the end of every classes, students' understanding are accessed by exams.
Related courses
- None
Prerequisites
None