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2025 (Current Year) Faculty Courses School of Life Science and Technology Undergraduate major in Life Science and Technology

LST Basic Laboratory and Exercise III B

Academic unit or major
Undergraduate major in Life Science and Technology
Instructor(s)
Nobuhiro Nakamura / Hiroshi Ichinose / Yuriko Osakabe / Shoen Kume / Naohiko Koshikawa / Atsushi Kawakami / Mie Shimojima / Nobuaki Shiraki / Takashi Suzuki / Mikiko Tanaka / Takashi Hirasawa / Akira Kato / Susumu Kajiwara / Shunichiro Ogura / Kanami Orihara / Junji Hirota / Shinji Masuda / Hiroshi Kimura / Kan Tanaka / Keisuke Yoshida / Tomoaki Matsuura / Kosuke Fujishima / Shawn Mcglynn / Keiji Inohaya / Satoshi Hara / Xinyue Chen / Ayumi Nagashima / Tetsuo Iwata / Nobuaki Funahashi / Kaisei Maeda / Satoshi Kidokoro / Toru Kawanishi / Kumi Kuroda / Shouta Nonoyama / Natsuki Osaka / Kseniia Prokofeva / Takeo Saneyoshi / Tokiwa Yamasaki / Yasunori Aizawa
Class Format
Exercise/Experiment (Face-to-face)
Media-enhanced courses
-
Day of week/Period
(Classrooms)
5-9 Mon (緑が丘3号館学生実験室) / 5-9 Thu (緑が丘3号館学生実験室)
Class
B
Course Code
LST.A253
Number of credits
012
Course offered
2025
Offered quarter
2Q
Syllabus updated
Oct 3, 2025
Language
Japanese

Syllabus

Course overview and goals

This course aims at learning principles and methods for basic experiments in biochemistry, which are required for conducting research in the laboratory.

Course description and aims

By the end of this course, students will be able to:
1) Conduct basic biochemical experiments required for research in the laboratory.
2) Solve problems when in trouble with basic biochemical experiments.
3) Describe the aim, results, and discussion of biochemical experiments clearly.

Keywords

Biochemistry

Competencies

  • Specialist skills
  • Intercultural skills
  • Communication skills
  • Critical thinking skills
  • Practical and/or problem-solving skills

Class flow

Students start experiments after the guidance on the flow of the experiments in the first ~30 min in each class. After the experiment, a report is to be submitted by the due date specified for each class.

Course schedule/Objectives

Course schedule Objectives
Class 1

Basic biochemical experiments 1: preparation of reagents, use of pH meter

Exercise and experimental report

Class 2

Basic biochemical experiments 2: centrifugation

Exercise and experimental report

Class 3

Protein experiments 1: extraction, separation, and quantification

Exercise and experimental report

Class 4

Protein experiments 2: electrophoresis, CBB stain, and electrotransfer to nylon membrane

Exercise and experimental report

Class 5

Protein experiments 3: western blotting and immunostaining

Exercise and experimental report

Class 6

Animal development 1: early development

Exercise and experimental report

Class 7

Animal development 2: cell differentiation and organ formation

Exercise and experimental report

Class 8

Microorganisms and phages 1: phage quantification

Exercise and experimental report

Class 9

Microorganisms and phages 2: bacterial phage infection and Gram staining

Exercise and experimental report

Class 10

Light microscopy of culture cells 1: phase-contrast microscopy of cell division, fluorescence staining

Exercise and experimental report

Class 11

Light microscopy of culture cells 2: fluorescence microscopy of cell division, cell death, and organelles

Exercise and experimental report

Class 12

Analysis of lipids and organelles 2: photosynthetic reaction of chloroplasts

Exercise and experimental report

Class 13

Analysis of lipids and organelles 1: extraction and separation of lipids from plants

Exercise and experimental report

Class 14

Analysis of lipids and organelles 3: isolation and functional analysis of mitochondria

Exercise and experimental report

Study advice (preparation and review)

To enhance effective learning, students are encouraged to spend a certain length of time outside of class on preparation and review (including for assignments), as specified by the Tokyo Institute of Technology Rules on Undergraduate Learning (東京工業大学学修規程) and the Tokyo Institute of Technology Rules on Graduate Learning (東京工業大学大学院学修規程), for each class.
They should do so by referring to textbooks and other course material.

Textbook(s)

An original textbook edited by the School of Life Science and Technology

Reference books, course materials, etc.

Handouts will be distributed as necessary.

Evaluation methods and criteria

Experimental training, report, exercise

Related courses

  • LST.A203 : Biochemistry I
  • LST.A218 : Biochemistry II
  • LST.A251 : LST Basic Laboratory and Exercise I
  • LST.A252 : LST Basic Laboratory and Exercise II
  • LST.A254 : LST Basic Laboratory and Exercise IV

Prerequisites

None