2024 Faculty Courses School of Life Science and Technology Department of Life Science and Technology Graduate major in Life Science and Technology
Biomolecular Analysis
- Academic unit or major
- Graduate major in Life Science and Technology
- Instructor(s)
- Toshiya Osada / Yoshitaka Ishii / Satoshi Okada / Kayo Nozawa / Toru Kondo / Takao Yasui
- Class Format
- Lecture (Livestream)
- Media-enhanced courses
- -
- Day of week/Period
(Classrooms) - 3-4 Mon / 3-4 Thu
- Class
- -
- Course Code
- LST.A501
- Number of credits
- 200
- Course offered
- 2024
- Offered quarter
- 1Q
- Syllabus updated
- Mar 14, 2025
- Language
- English
Syllabus
Course overview and goals
This course is organized into five parts.
The first part describes the various advantages obtained by performing biomolecular measurements in small spaces from a physical principle standpoint and discusses the new developments in biomolecular measurement research brought about by small spaces.
The second part introduces nuclear magnetic resonance (NMR) spectroscopy.
The third part introduces the single-molecule spectroscopy (SMS), used for the spectroscopic analysis of molecular dynamics in biological systems.
The forth part describes basic principle of magnetic resonance imaging (MRI) and its application to molecular imaging.
The firth part of the course deals with an AFM (atomic force microscopy). The AFM has become a powerful tool to study biological samples not only for imaging at the molecular level but also for measuring their mechanical properties. The course begins with an overview of AFM and then goes to applications of AFM to the study of the biological materials.
The last part describesX-ray crystallography and cryo-electron microscopy has provided important insights into macromolecules such as drug-protein complexes and viruses. This lecture summarizes the outline of the principles and applications of these technologies.
Course description and aims
By the end of this course, students will be able to:
1) gain understanding of the basic principles of AFM.
2) learn the broad applications of AFM in biological fields
3) understand various biomolecular measurements facilitated by technological innovations related to micro- and nanotechnology, such as microstructure analysis, microfabrication, and molecular/material synthesis, on the micro- and nanometer scales.
4) learn theory and applications of NMR spectroscopy
5) understand theory and application of MRI
6) understand basics and applications of SMS
7)Understanding the X-ray crystallographic and cryo-electron microscopic methods. Familialize with analyses of the three-dimensional structure and complex formation of biomolecules using the methods.
Keywords
AFM, small spaces, micro- and nanotechnology,, Laser spectroscopy, mass spectrometry, nuclear magnetic resonance, NMR, MRI, single-molecule spectroscopy, microspectroscopy
Competencies
- Specialist skills
- Intercultural skills
- Communication skills
- Critical thinking skills
- Practical and/or problem-solving skills
Class flow
The class starts with reviews of previous class. Towards the end of class, students are often given exercise problems related to the lecture given that day to solve. To prepare for the class, students should read the course schedule section and check what topics will be covered. Required learning should be completed outside the classroom for preparation and review purposes.
Classes will be basically done in English, but if necessary we will provide supplementary explanation in Japanese.
Classes will be held online using Zoom.
Course schedule/Objectives
| Course schedule | Objectives | |
|---|---|---|
| Class 1 | Biomolecular measurements in small spaces; (1) fluid behavior in small spaces | Understanding molecular vibration including normal mode |
| Class 2 | Biomolecular measurements in small spaces; (2) analytical methods utilizing small spaces | Understanding biological analysis, chemical analysis, electrophoresis |
| Class 3 | NMR spectroscopy: (1) Introduction to NMR spectroscopy | Understanding of basic theory and applications of NMR spectroscopy |
| Class 4 | NMR spectroscopy: (2) Classical theory and 1D NMR | Understanding of classical theory of NMR spectroscopy and 1D NMR |
| Class 5 | NMR spectroscopy: (3) 2-3D NMR and applications to small molecules, proteins, and imaging | Understanding of 2-3D NMR and advanced |
| Class 6 | Single-molecule spectroscopy: (1) Microspectroscopy at the single-molecule level | Understanding of microspectroscopy at the single-molecule level |
| Class 7 | Single-molecule spectroscopy: (2) Spectroscopic analysis of fluctuations | Understanding of spectroscopic measurement and analysis of fluctuations |
| Class 8 | Single-molecule spectroscopy: (3) Applications to biological systems | Understanding of applications of single-molecule spectroscopy |
| Class 9 | Magnetic resonance imaging (MRI): (1) Introduction | Understanding basic principle of MRI |
| Class 10 | Magnetic resonance imaging (MRI): (2) Contrast agents and molecular imaging | Understanding application of MRI to molecular imaging |
| Class 11 | X-ray crystallography and cryo-electron microscopy: (1) Introduction and basic theory | Understanding of the principle of diffraction and imaging |
| Class 12 | X-ray crystallography and cryo-electron microscopy: (2) Application to biomolecules | Understanding of applications to macromolecules and their impact |
| Class 13 | Introduction to atomic force microscopy(AFM) | AFM instrumentation, Surface forces, Contact mechanics |
| Class 14 | AFM based single-molecule force spectroscopy | Explain the principle and the application of AFM to single molecule. |
Study advice (preparation and review)
To enhance effective learning, students are encouraged to spend approximately 100 minutes preparing for class and another 100 minutes reviewing class content afterwards (including assignments) for each class.
They should do so by referring to textbooks and other course material.
Textbook(s)
No textbook is set.
Reference books, course materials, etc.
Handouts will be distributed at the beginning of class when necessary and elaborated on using PowerPoint slides.
Evaluation methods and criteria
Students' knowledge of basic matters, understanding on essential significance and abilities to apply them to problems will be assessed. No midterm and final exams.
Related courses
- LST.A409 ： Physical Biology of the Cell
Prerequisites
Students must have successfully completed Physical Chemistry I, II, and III (number) or have equivalent knowledge.