2024 Faculty Courses School of Life Science and Technology Undergraduate major in Life Science and Technology
LST Basic Laboratory and Exercise IV B
- Academic unit or major
- Undergraduate major in Life Science and Technology
- Instructor(s)
- Yuriko Osakabe / Yumiko Igarashi / Akio Kitao / Toshiaki Fukui / Yuki Yamaguchi / Masaaki Wachi / Yasunori Aizawa / Yoh-Ichi Tagawa / Hitoshi Nakatogawa / Rie Yatsunami / Masaki Kajikawa / Tetsuya Kadonosono / Kazunori Tachibana / Hiroshi Iwasaki / Naonobu Fujita / Tetsuya Kitaguchi / Fumi Kano / Kosuke Fujishima / Noritaka Iwai / Eiichi Okumura / Izumi Orita / Shinya Kaneko / Satoshi Sakamoto / Toshiaki Tanaka / Hideo Tsubouchi / Toshiaki Fukushima / Bo Zhu / Daiki Nakatsu / Junichi Yamamoto / Takanobu Yasuda / Satoshi Kidokoro / Eiry Kobatake / Mako Kamiya / Nobuaki Shiraki / Taiko To / Keisuke Mochida
- Class Format
- Exercise/Experiment (Face-to-face)
- Media-enhanced courses
- -
- Day of week/Period
(Classrooms) - 5-9 Mon / 5-9 Thu
- Class
- B
- Course Code
- LST.A254
- Number of credits
- 012
- Course offered
- 2024
- Offered quarter
- 3Q
- Syllabus updated
- Mar 17, 2025
- Language
- Japanese
Syllabus
Course overview and goals
This course aims at learning principles and methods for basic experiments in molecular biology, which are required for conducting research in the laboratory.
Course description and aims
By the end of this course, students will be able to:
1) Conduct basic molecular biological experiments required for research in the laboratory.
2) Solve problems when in trouble with basic molecular biological experiments.
3) Describe the aim, results, and discussion of molecular biological experiments clearly.
Keywords
Molecular biology
Competencies
- Specialist skills
- Intercultural skills
- Communication skills
- Critical thinking skills
- Practical and/or problem-solving skills
Class flow
Students start experiments after the guidance on the flow of the experiments in the first ~30 min in each class.
Course schedule/Objectives
| Course schedule | Objectives | |
|---|---|---|
| Class 1 | Cultivation of microorganisms 1 | Exercise and experimental report |
| Class 2 | Cultivation of microorganisms 2 | Exercise and experimental report |
| Class 3 | Transformation of Escherichia coli 1 | Exercise and experimental report |
| Class 4 | Transformation of Escherichia coli 2 | Exercise and experimental report |
| Class 5 | Transformation of Escherichia coli 3 | Exercise and experimental report |
| Class 6 | Analysis of DNA topology and higher order structure 1 | Exercise and experimental report |
| Class 7 | Analysis of DNA topology and higher order structure 2 | Exercise and experimental report |
| Class 8 | Analysis of DNA topology and higher order structure 3 | Exercise and experimental report |
| Class 9 | Analysis of mRNA expression 1 | Exercise and experimental report |
| Class 10 | Analysis of mRNA expression 2 | Exercise and experimental report |
| Class 11 | Analysis of mRNA expression 3 | Exercise and experimental report |
| Class 12 | Analysis of gene mutation conferring resistance to antibiotics 1 | Exercise and experimental report |
| Class 13 | Analysis of gene mutation conferring resistance to antibiotics 2 | Exercise and experimental report |
| Class 14 | Analysis of gene mutation conferring resistance to antibiotics 3 | Exercise and experimental report |
Study advice (preparation and review)
To enhance effective learning, students are encouraged to spend a certain length of time outside of class on preparation and review (including for assignments), as specified by the Tokyo Institute of Technology Rules on Undergraduate Learning (東京工業大学学修規程) and the Tokyo Institute of Technology Rules on Graduate Learning (東京工業大学大学院学修規程), for each class.
They should do so by referring to textbooks and other course material.
Textbook(s)
An original textbook edited by the School of Life Science and Technology
Reference books, course materials, etc.
Handouts will be distributed as necessary.
Evaluation methods and criteria
Experimental training, report
Related courses
- LST.A208 ： Molecular Biology I
- LST.A213 ： Molecular Biology II
Prerequisites
None
Other
None