2024 Faculty Courses School of Life Science and Technology Creative process courses
Processes for creation in science and technology【School of Life Science and Technology】
- Academic unit or major
- Creative process courses
- Instructor(s)
- Yuki Yamaguchi / Hideki Taguchi / Hiroshi Kimura / Satoshi Murakami / Kohji Seio / Akihiro Ohkubo
- Class Format
- Lecture (Face-to-face)
- Media-enhanced courses
- -
- Day of week/Period
(Classrooms) - 5-6 Tue
- Class
- -
- Course Code
- XLS.P101
- Number of credits
- 100
- Course offered
- 2024
- Offered quarter
- 2Q
- Syllabus updated
- Mar 14, 2025
- Language
- Japanese
Syllabus
Course overview and goals
How the green fluorescence protein (GFP) emits light is discussed from various perspectives such as physical chemistry, organic chemistry, and biochemistry to introduce the idea that structure makes function. Through active learning with the use of molecular models and tablet PCs, students are guided to an intuitive understanding of the flow of genetic information (the central dogma), protein structure and folding, the mechanism by which chromophore emits fluorescence, the technology behind fluorescence microscopy and so on, leading to a higher level of interest in life science and technology.
Course description and aims
By the end of this course, students will be able to:
1. understand how GFP emits light from various perspectives such as physical chemistry, organic chemistry, biochemistry, and structural biology.
2. understand, at the basic level, and explain the structure of GFP that is critical for emitting light, the flow of genetic information that makes protein, and technology behind fluorescence microscopy.
Keywords
Green fluorescent protein (GFP), the central dogma, protein folding, fluorescence microscopy
Competencies
- Specialist skills
- Intercultural skills
- Communication skills
- Critical thinking skills
- Practical and/or problem-solving skills
Class flow
Both lecture and exercise are conducted in parallel. Students are asked to solve problems using molecular models, tablet PCs, and other tools in the course.
Course schedule/Objectives
| Course schedule | Objectives | |
|---|---|---|
| Class 1 | How GFP emits light #1: With a focus on various organic molecules found in nature, the mechanism of fluorescence emission is explained at the atomic level. | Students must be able to explain the physicochemical mechanism by which various organic molecules emit light. |
| Class 2 | How GFP emits light #2: The physicochemical properties of p-hydroxybenzylidene imidazolinone, the chromophore of GFP, are explained in order to show exactly how GFP emits fluorescence. | Students must be able to describe the molecular structure of the chromophore of GFP and to explain the mechanism of fluorescence emission in detail. |
| Class 3 | rotein structure underlying fluorescence emission of GFP #1: The entire molecular structure of GFP is examined at the atomic level from the perspective of structural biology. | Students must be able to describe the characteristics of the tertiary structure of GFP. |
| Class 4 | Protein structure underlying fluorescence emission of GFP #2: The process of protein folding, which allows the formation of a specific tertiary structure from its linear sequence, is explained using GFP as an example. | Students must be able to describe the process of protein folding using GFP as an example. |
| Class 5 | The principle of fluorescence microscopy: The technological basis for the observation of fluorescence emitted from GFP and its variants is explained. | Students must be able to explain the principle of fluorescence microscopy. |
| Class 6 | How the genetic information of GFP is replicated: How the genetic information for GFP is securely stored in the cell nucleus and maintained over generations will be explained. | Student must be able to explain the structure of DNA double helix at the atomic level and the molecular basis for its maintenance over generations. |
| Class 7 | Cell biological study using GFP: By using GFP, the pattern of gene expression, subcellular localization of a protein and biochemical processes in the cell can be visualized. Various applications of GFP are presented. | Students must be able to raise some examples on cell biological study using GFP. |
Study advice (preparation and review)
To enhance effective learning, students need to carry out preparation and review following instructors' instructions.
Textbook(s)
None required.
Reference books, course materials, etc.
None required.
Evaluation methods and criteria
Assessment is based on the quality of in-class assignments. There is no end-term examination. Full attendance is compulsory.
Related courses
- ENT.B101(LAS.F101) ： Frontiers of science and technology
Prerequisites
There are no prerequisites, but it is recommended that students have successfully completed Frontiers of Science and Technology.