2023 Faculty Courses School of Life Science and Technology Undergraduate major in Life Science and Technology
LST Basic Laboratory and Exercise III D
- Academic unit or major
- Undergraduate major in Life Science and Technology
- Instructor(s)
- Akio Kitao / Hiroshi Ichinose / Yuriko Osakabe / Shoen Kume / Naohiko Koshikawa / Atsushi Kawakami / Mie Shimojima / Nobuaki Shiraki / Takashi Suzuki / Mikiko Tanaka / Nobuhiro Nakamura / Takashi Hirasawa / Akira Kato / Susumu Kajiwara / Shunichiro Ogura / Kanami Orihara / Junji Hirota / Shinji Masuda / Hiroshi Kimura / Kan Tanaka / Keisuke Yoshida / Keiko Nonomura / Tomoaki Matsuura / Kosuke Fujishima / Shawn Mcglynn / Keiji Inohaya / Daisuke Sakano / Satoshi Hara / Koichi Hori / Xinyue Chen / Ayumi Nagashima / Yuko Sato / Tetsuo Iwata / Nobuaki Funahashi / Kaisei Maeda / Satoshi Kidokoro / Toru Kawanishi
- Class Format
- Exercise/Experiment (Face-to-face)
- Media-enhanced courses
- -
- Day of week/Period
(Classrooms) - 5-9 Mon (緑が丘3号館学生実験室) / 5-9 Thu (緑が丘3号館学生実験室)
- Class
- D
- Course Code
- LST.A253
- Number of credits
- 012
- Course offered
- 2023
- Offered quarter
- 4Q
- Syllabus updated
- Jul 8, 2025
- Language
- Japanese
Syllabus
Course overview and goals
This course aims at learning principles and methods for basic experiments in biochemistry, which are required for conducting research in the laboratory.
Course description and aims
By the end of this course, students will be able to:
1) Conduct basic biochemical experiments required for research in the laboratory.
2) Solve problems when in trouble with basic biochemical experiments.
3) Describe the aim, results, and discussion of biochemical experiments clearly.
Keywords
Biochemistry
Competencies
- Specialist skills
- Intercultural skills
- Communication skills
- Critical thinking skills
- Practical and/or problem-solving skills
Class flow
Students start experiments after the guidance on the flow of the experiments in the first ~30 min in each class. After the experiment, a report is to be submitted by the due date specified for each class.
(Attention!) Due to the COVID-19 countermeasures, experiments in 2022 will be conducted according to the schedule that will be distributed/noticed at the first session. Experiments will be conducted in two groups, and Zoom may be used in some cases.
Course schedule/Objectives
| Course schedule | Objectives | |
|---|---|---|
| Class 1 | Basic biochemical experiments 1: preparation of reagents, use of pH meter | Exercise and experimental report |
| Class 2 | Basic biochemical experiments 2: centrifugation | Exercise and experimental report |
| Class 3 | Protein experiments 1: extraction, separation, and quantification | Exercise and experimental report |
| Class 4 | Protein experiments 2: electrophoresis, CBB stain, and electrotransfer to nylon membrane | Exercise and experimental report |
| Class 5 | Protein experiments 3: western blotting and immunostaining | Exercise and experimental report |
| Class 6 | Analysis of lipids and organelles 1: extraction and separation of lipids from plants | Exercise and experimental report |
| Class 7 | Analysis of lipids and organelles 2: photosynthetic reaction of chloroplasts | Exercise and experimental report |
| Class 8 | Analysis of lipids and organelles 3: isolation and functional analysis of mitochondria | Exercise and experimental report |
| Class 9 | Microorganisms and phages 1: phage quantification | Exercise and experimental report |
| Class 10 | Microorganisms and phages 2: bacterial phage infection and Gram staining | Exercise and experimental report |
| Class 11 | Light microscopy of culture cells 1: phase-contrast microscopy of cell division, fluorescence staining | Exercise and experimental report |
| Class 12 | Light microscopy of culture cells 2: fluorescence microscopy of cell division, cell death, and organelles | Exercise and experimental report |
| Class 13 | Animal development 1: early development | Exercise and experimental report |
| Class 14 | Animal development 2: cell differentiation and organ formation | Exercise and experimental report |
Study advice (preparation and review)
To enhance effective learning, students are encouraged to spend a certain length of time outside of class on preparation and review (including for assignments), as specified by the Tokyo Institute of Technology Rules on Undergraduate Learning (東京工業大学学修規程) and the Tokyo Institute of Technology Rules on Graduate Learning (東京工業大学大学院学修規程), for each class.
They should do so by referring to textbooks and other course material.
Textbook(s)
An original textbook edited by the School of Life Science and Technology
Reference books, course materials, etc.
Handouts will be distributed as necessary.
Evaluation methods and criteria
Experimental training, report, exercise
Related courses
- LST.A203 ： Biochemistry I
- LST.A218 ： Biochemistry II
- LST.A251 ： LST Basic Laboratory and Exercise I
- LST.A252 ： LST Basic Laboratory and Exercise II
- LST.A254 ： LST Basic Laboratory and Exercise IV
Prerequisites
None