2020 Faculty Courses School of Life Science and Technology Undergraduate major in Life Science and Technology

LST Basic Laboratory and Exercise III B

Academic unit or major: Undergraduate major in Life Science and Technology
Instructor(s): Hiroshi Ichinose / Hiroyuki Ohta / Shoen Kume / Atsushi Kawakami / Mie Shimojima / Nobuaki Shiraki / Takashi Suzuki / Mikiko Tanaka / Nobuhiro Nakamura / Takashi Hirasawa / Akira Kato / Shunichiro Ogura / Kanami Orihara / Junji Hirota / Shinji Masuda / Hiroshi Kimura / Kan Tanaka / Toru Hisabori / Keiji Inohaya / Daisuke Sakano / Hidenori Nishihara / Satoshi Hara / Koichi Hori / Kazuhiko Miyanaga / Xinyue Chen / Ayumi Nagashima / Yuko Sato / Yuki Kobayashi / Keisuke Yoshida
Class Format: Exercise/Experiment
Media-enhanced courses: -
Day of week/Period (Classrooms): 2-8 Intensive (緑が丘3号館学生実験室)
Class: B
Course Code: LST.A253
Number of credits: 012
Course offered: 2020
Offered quarter: 2Q
Syllabus updated: Jul 10, 2025
Language: Japanese

Syllabus

Course overview and goals

This course aims at learning principles and methods for basic experiments in biochemistry, which are required for conducting research in the laboratory.

Course description and aims

By the end of this course, students will be able to:
1) Conduct basic biochemical experiments required for research in the laboratory.
2) Solve problems when in trouble with basic biochemical experiments.
3) Describe the aim, results, and discussion of biochemical experiments clearly.

Keywords

Biochemistry

Competencies

Specialist skills
Intercultural skills
Communication skills
Critical thinking skills
Practical and/or problem-solving skills

Class flow

Students start experiments after the guidance on the flow of the experiments in the first ~30 min in each class.

Course schedule/Objectives

	Course schedule	Objectives
Class 1	Basic biochemical experiments 1: preparation of reagents, use of pH meter	Exercise and experimental report
Class 2	Basic biochemical experiments 2: centrifugation	Exercise and experimental report
Class 3	Protein experiments 1: extraction, separation, and quantification	Exercise and experimental report
Class 4	Protein experiments 2: electrophoresis, CBB stain, and electrotransfer to nylon membrane	Exercise and experimental report
Class 5	Protein experiments 3: western blotting and immunostaining	Exercise and experimental report
Class 6	Analysis of lipids and organelles 1: extraction and separation of lipids from plants	Exercise and experimental report
Class 7	Analysis of lipids and organelles 2: photosynthetic reaction of chloroplasts	Exercise and experimental report
Class 8	Analysis of lipids and organelles 3: isolation and functional analysis of mitochondria	Exercise and experimental report
Class 9	Microorganisms and phages 1: phage quantification	Exercise and experimental report
Class 10	Microorganisms and phages 2: bacterial phage infection and Gram staining	Exercise and experimental report
Class 11	Light microscopy of culture cells 1: phase-contrast microscopy of cell division, fluorescence staining	Exercise and experimental report
Class 12	Light microscopy of culture cells 2: fluorescence microscopy of cell division, cell death, and organelles	Exercise and experimental report
Class 13	Animal development 1: early development	Exercise and experimental report
Class 14	Animal development 2: cell differentiation and organ formation	Exercise and experimental report

Study advice (preparation and review)

Textbook(s)

An original textbook edited by the School of Life Science and Technology

Reference books, course materials, etc.

Handouts will be distributed as necessary.

Evaluation methods and criteria

Experimental training, report, exercise

Related courses

LST.A203 ： Biochemistry I
LST.A218 ： Biochemistry II
LST.A251 ： LST Basic Laboratory and Exercise I
LST.A252 ： LST Basic Laboratory and Exercise II
LST.A254 ： LST Basic Laboratory and Exercise IV

Prerequisites

None