2020 Faculty Courses School of Life Science and Technology Undergraduate major in Life Science and Technology
LST Basic Laboratory and Exercise I B
- Academic unit or major
- Undergraduate major in Life Science and Technology
- Instructor(s)
- Yoshitaka Ishii / Takehiko Itoh / Takafumi Ueno / Toshiaki Kamachi / Eiry Kobatake / Akio Kitao / Makio Tokunaga / Satoshi Murakami / Toshiya Osada / Nobuhiro Hayashi / Masayasu Mie / Eizo Miyashita / Takuji Yamada / Noriyuki Asakura / Hideki Taguchi / Masaaki Fujii / Hiroyuki Akama / Shun-Ichi Ishiuchi / Satoshi Abe / Yuma Ito / Ui Okada / Rei Kajitani / Takayuki Kamihara / Duy Phuoc Tran / Kimitoshi Denda / Tadaomi Furuta / Yasumasa Mashimo / Tatsuya Niwa / Keisuke Hirata / Hidehiro Ito
- Class Format
- Exercise/Experiment
- Media-enhanced courses
- -
- Day of week/Period
(Classrooms) - Intensive (緑が丘3号館学生実験室)
- Class
- B
- Course Code
- LST.A251
- Number of credits
- 012
- Course offered
- 2020
- Offered quarter
- 3Q
- Syllabus updated
- Jul 10, 2025
- Language
- Japanese
Syllabus
Course overview and goals
This course aims at learning the experimental principles and methods of physical chemistry and related knowledge that are required to carry out bachelor thesis research.
Course description and aims
By the end of this course, students will be able to:
1) Conduct basic experiments required for bachelor thesis research.
2) Devise a troubleshooting method when the experiment fails.
3) Clearly note the purpose, results, and discussion of the experiment.
Keywords
Physical chemistry
Competencies
- Specialist skills
- Intercultural skills
- Communication skills
- Critical thinking skills
- Practical and/or problem-solving skills
Class flow
The experiment will be conducted after explaining the experiment in about 30 minutes.
Course schedule/Objectives
| Course schedule | Objectives | |
|---|---|---|
| Class 1 | Basics and usage of spectrophotometer I | |
| Class 2 | Basics and usage of spectrophotometer II | |
| Class 3 | Separation and purification of proteins I | |
| Class 4 | Separation and purification of proteins II | |
| Class 5 | Separation and purification of proteins III | |
| Class 6 | Kinetic analysis of enzyme reaction I | |
| Class 7 | Kinetic analysis of enzyme reaction II | |
| Class 8 | Kinetic analysis of enzyme reaction III | |
| Class 9 | Analysis of protein-ligand binding by equilibrium dialysis | |
| Class 10 | Measurement of acid dissociation constant | |
| Class 11 | Quantification of diffusion coefficient and Avogadro number by Brownian motion measurement | |
| Class 12 | Computer analysis of biomolecules I | |
| Class 13 | Computer analysis of biomolecules II | |
| Class 14 | Computer analysis of biomolecules III |
Study advice (preparation and review)
Textbook(s)
Original text created by the the School of Life Science and Technology
Reference books, course materials, etc.
No reference book is specified.
Handouts will be distributed as needed.
Evaluation methods and criteria
The grade is evaluated by the attitude toward the experiment, the degree of skill of the experiment operation technique, and the experiment report.
Related courses
- LST.A222 ： LST Basic Laboratory II
- LST.A223 ： LST Basic Laboratory III
- LST.A224 ： LST Basic Laboratory IV
Prerequisites
No special requirements